Separation and mapping of chromosomes of parasitic protozoa.
نویسندگان
چکیده
Many protozoan parasites represent an important group of human pathogens. Pulsed Field Gradient Gel Electrophoresis (PFGE) analysis has been an important tool for fundamental genetic studies of parasites like Trypanosoma, Leishmania, Giardia or the human malaria parasite Plasmodium falciparum. We present PFGE conditions allowing a high resolution separation of chromosomes ranging from 500 to 4000 kb within a two day electrophoresis run. In addition, we present conditions for separating large chromosomes (2000-6000 kb) within 36 hr. We demonstrate that the application of two dimensional PFGE (2D-PFGE) technique to parasite karyotypes is a very useful method for the analysis of dispersed gene families and comparative studies of the intrachromosomal genome organization.
منابع مشابه
Occurrence of parasitic protozoa in wild waterfowl in southern coastal Caspian sea lagoons
BACKGROUND: Protozoa are common in poultry and otherbirds and can cause mild to severe disease. OBJECTIVES: Thisstudy was concerned with the prevalence of parasitic protozoa inwild waterfowl in Caspian sea lagoons in the North of Iran.METHODS:Atotal of 293 waterfowl belonging to various generaincluding Tadorna tadorna (common shelduck),Aythya fuligula(Tufted duck), Aythya ferina (Common puchard...
متن کاملبررسی وجود استرس و التهاب عمومی در مبتلایان به آلودگیهای تکیاختهای رودهای در دو بیمارستان تهران
Background: Protozoa are unicellular eukaryotes. A type of intestinal protozoan pathogens is the cause of infection and serious injuries in human with or without clinical symptoms. It seems that parasitic infections as a stressor factor can affect on stress system activity and immune system function and sensitivity. The major purpose of this paper was to evaluate serum cortisol level and the ge...
متن کاملبررسی انگلهای مرتبط با بیماری های قلبی
Introduction: A wide variety of protozoa and helminthes could influence the heart directly or indirectly. The manifestations may lead to different warning signs with the most frequent myocardium and pericardium. The parasites related to the heart diseases are reviewed in the present study. Methods and Materials: This review article has been performed by searching cardiac disease, helminthes, a...
متن کاملMapping of QTLs Controlling Egg Quality on Chromosomes 6-8, Z and Three Linkage Groups in Chickens
Two breeds of chickens were used to develop reference family and detect QTLs affecting egg quality traits: cocks of a native Polish breed Green-Legged Partidgenous (GlP) and hens of Rhode Island Red (RIR). During the first 100 days of the laying period, the GlP hens lay about 40 eggs weighting on average 50 g, whilst the RIR flock laid 83 eggs with a mean weight of 60 g. QTL analysis of egg qua...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Memorias do Instituto Oswaldo Cruz
دوره 92 6 شماره
صفحات -
تاریخ انتشار 1997